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By Mark R. Marten, Tai Hyun Park, and Teruyuki Nagamune (Eds.)

ISBN-10: 0841219311

ISBN-13: 9780841219311

ISBN-10: 0841237964

ISBN-13: 9780841237964

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18. ; Cecchini, G. Arch. Biochem. Biophys. 1999, 369(2), 223232. 19. Donnelly, M . ; Millard, C. ; Clark, D. ; Chen, M . W. Appl. Biochem. Biotechnol. 1998, 70-72, 187-198. 20. ; Millard, C. ; Champion, K . ; Clark, D. ; Donnelly, M . I. Appl. Environ. Microbiol. 2001, 67, 148-154. 21. Hong, S. ; Lee, S. Y. J. Microbiol. Biotechnol. 2000, 10(4), 496-501. ; ACS Symposium Series; American Chemical Society: Washington, DC, 2002. ch004 Metabolic Control Analysis in Glutamate Synthetic Pathway: Experimental Sensitivity Analysis at a Key Branch Point Hiroshi Shimizu, Hisaya Tanaka, Akinori Nakato, Keisuke Nagahisa, and Suteaki Shioya* Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita Osaka 565-0871 Japan Experimental method for metabolic control analysis (MCA) was applied to investigation of a metabolic network of glutamate production by Corynebacterium glutamicum.

Millard, C. ; Chao, Y . ; Liao, J. ; Donnelly, M . I. Appl. Environ. Microbiol. 1996, 62, 1808-1810. 12. Flores, C. ; Gancedo, C. FEBS Lett. 1997, 412(3), 531-534. 13. Laivenieks, M . ; Zeikus, J. G. Appl. Environ. Microbiol. 1997, 63, 2273-2280. 14. ; Donnelly, M . I. Appl. Environ. Microbiol. 1997, 63, 2695-2701. 15. ; Harris, B. ; Donnelly, M . I. Appl. Biochem. Biotechnol. 1997, 63-65, 153-158. 16. Hong, S. , Lee, S. Bioeng. 2001, 74(2), 89-95. 17. ; Villadsen, J. ; Plenum press: N Y , 1994, pp 97-161.

Cells were disrupted by sonication and the supernatant was used as cell extract. The enzymes activities were assayed photometrically at 3L5°C in 1 mL of a reaction mixture. 3mM isocitrare. The ICDH activity was measured from increase in the absorption at 340 nm. (11). 25mM N A D P H , 10 m M 2-oxoglutarate. The G D H activity was measured from decrease in the absorption at 340 nm. (10). 3m M thiamine pyrophosphate, I m M 2-oxoglutarate, 3mM cysteine, 5mM M g C l , and I m M 3-acetylpyridine adenine dinucreotide, the last of which was used instead of N A D .

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Biological Systems Engineering by Mark R. Marten, Tai Hyun Park, and Teruyuki Nagamune (Eds.)

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